PRTN3 anticorps

N° du produit ABIN190718

L’anticorps Lapin Polyclonal anti-PRTN3 a été validé pour WB. Il convient pour détecter PRTN3 dans des échantillons de Nicotiana tabacum et Potato.

Aperçu rapide pour PRTN3 anticorps (ABIN190718)

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Reactivité: Nicotiana tabacum, Potato

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRTN3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PRTN3 anticorps

Specificité: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 35, 34 / 32 and 34 kDa

Purification: affinity purified

Immunogène: purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990, van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Isotype: IgG

Information d'application

Indications d'application: Recommended Dilution: 8 ug/mL with standard ECL (WB).

Commentaires:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Détails sur PRTN3

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Autre désignation: PR-3

Sujet: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Poids moléculaire: expected: 35 kDa, 34 kDa, apparent: 32 and 34 kDa

UniProt: P08252, P24091